Using random forests to uncover the predictive power of distance-varying cell interactions in tumor microenvironments
- Author(s)
- VanderDoes, J; Marceaux, C; Yokote, K; Asselin-Labat, ML; Rice, G; Hywood, JD;
- Details
- Publication Year 2024-06-14,Volume 20,Issue #6,Page e1011361
- Journal Title
- PLoS Computational Biology
- Abstract
- Tumor microenvironments (TMEs) contain vast amounts of information on patient's cancer through their cellular composition and the spatial distribution of tumor cells and immune cell populations. Exploring variations in TMEs between patient groups, as well as determining the extent to which this information can predict outcomes such as patient survival or treatment success with emerging immunotherapies, is of great interest. Moreover, in the face of a large number of cell interactions to consider, we often wish to identify specific interactions that are useful in making such predictions. We present an approach to achieve these goals based on summarizing spatial relationships in the TME using spatial K functions, and then applying functional data analysis and random forest models to both predict outcomes of interest and identify important spatial relationships. This approach is shown to be effective in simulation experiments at both identifying important spatial interactions while also controlling the false discovery rate. We further used the proposed approach to interrogate two real data sets of Multiplexed Ion Beam Images of TMEs in triple negative breast cancer and lung cancer patients. The methods proposed are publicly available in a companion R package funkycells.
- Publisher
- PLOS
- Research Division(s)
- Personalised Oncology
- PubMed ID
- 38875302/
- Publisher's Version
- https://doi.org/10.1371/journal.pcbi.1011361
- Open Access at Publisher's Site
https://doi.org/10.1371/journal.pcbi.1011361- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2024-06-24 11:30:02
Last Modified: 2024-06-24 11:40:28