Protocol for generation of and high-throughput drug testing with patient-derived colorectal cancer organoids
- Author(s)
- Tan, T; Mouradov, D; Gibbs, P; Sieber, OM;
- Details
- Publication Year 2024-05-28,Volume 5,Issue #2,Page 103090
- Journal Title
- STAR Protocols
- Abstract
- Drug sensitivity testing of patient-derived tumor organoids (PDTOs) is a promising tool for personalizing cancer treatment. Here, we present a protocol for generation of and high-throughput drug testing with PDTOs. We describe detailed steps for PDTO establishment from colorectal cancer tissues, preparation of PDTOs for high-throughput drug testing, and quantification of drug testing results using image analysis. This protocol provides a standardized workflow for PDTO testing of standard-of-care therapies, along with exploring the activity of new agents, for translational research. For complete details on the use and execution of this protocol, please refer to Tan et al.(1).
- Publisher
- Elsevier
- Keywords
- cancer; cell culture; high throughput screening; microscopy; organoids
- Research Division(s)
- Personalised Oncology
- PubMed ID
- 38809757
- Publisher's Version
- https://doi.org/10.1016/j.xpro.2024.103090
- Open Access at Publisher's Site
https://doi.org/10.1016/j.xpro.2024.103090- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2024-08-23 02:54:17
Last Modified: 2024-08-23 03:07:29