Deep mutational scanning of CYP2C19 in human cells reveals a substrate specificity-abundance tradeoff
- Author(s)
- Boyle, GE; Sitko, K; Galloway, JG; Haddox, HK; Bianchi, AH; Dixon, A; Wheelock, MK; Vandi, AJ; Wang, ZR; Thomson, RES; Garge, RK; Rettie, AE; Rubin, AF; Geck, RC; Gillam, EMJ; DeWitt, WS; Matsen, FAth; Fowler, DM;
- Journal Title
- Genetics
- Publication Type
- Sep 25 epub ahead of print
- Abstract
- The Cytochrome P450s (CYPs) enzyme family metabolizes ∼80% of small molecule drugs. Variants in CYPs can substantially alter drug metabolism, leading to improper dosing and severe adverse drug reactions. Due to low sequence conservation, predicting variant effects across CYPs is challenging. Even closely related CYPs like CYP2C9 and CYP2C19, which share 92% amino acid sequence identity, display distinct phenotypic properties. Using Variant Abundance by Massively Parallel sequencing (VAMP-seq), we measured the steady-state protein abundance of 7,660 single amino acid variants in CYP2C19 expressed in cultured human cells. Our findings confirmed critical positions and structural features essential for CYP function and revealed how variants at conserved positions influence abundance. We jointly analyzed 4,670 variants whose abundance was measured in both CYP2C19 and CYP2C9, finding that the homologs have different variant abundances in substrate recognition sites within the hydrophobic core. We also measured the abundance of all single and some multiple WT amino acid exchanges between CYP2C19 and CYP2C9. While most exchanges had no effect, substitutions in substrate recognition site 4 (SRS4) reduced abundance in CYP2C19. Double and triple mutants showed distinct interactions, highlighting a region that points to differing thermodynamic properties between the two homologs. These positions are known contributors to substrate specificity, suggesting an evolutionary tradeoff between stability and enzymatic function. Finally, we analyzed 368 previously unannotated human variants, finding that 43% had decreased abundance. By comparing variant effects between these homologs, we uncovered regions underlying their functional differences, advancing our understanding of this versatile family of enzymes.
- Publisher
- Oxford Academic
- Keywords
- cytochrome P450; deep mutational scanning; substrate specificity; thermodynamic stability
- Research Division(s)
- Bioinformatics
- PubMed ID
- 39319420
- Publisher's Version
- https://doi.org/10.1093/genetics/iyae156
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2024-10-04 10:44:49
Last Modified: 2024-10-04 10:51:24