The Circulating Proteome─Technological Developments, Current Challenges, and Future Trends
- Author(s)
- Geyer, PE; Hornburg, D; Pernemalm, M; Hauck, SM; Palaniappan, KK; Albrecht, V; Dagley, LF; Moritz, RL; Yu, X; Edfors, F; Vandenbrouck, Y; Mueller-Reif, JB; Sun, Z; Brun, V; Ahadi, S; Omenn, GS; Deutsch, EW; Schwenk, JM;
- Journal Title
- Journal of Proteome Research
- Publication Type
- 31 Oct epub ahead of print
- Abstract
- Recent improvements in proteomics technologies have fundamentally altered our capacities to characterize human biology. There is an ever-growing interest in using these novel methods for studying the circulating proteome, as blood offers an accessible window into human health. However, every methodological innovation and analytical progress calls for reassessing our existing approaches and routines to ensure that the new data will add value to the greater biomedical research community and avoid previous errors. As representatives of HUPO's Human Plasma Proteome Project (HPPP), we present our 2024 survey of the current progress in our community, including the latest build of the Human Plasma Proteome PeptideAtlas that now comprises 4608 proteins detected in 113 data sets. We then discuss the updates of established proteomics methods, emerging technologies, and investigations of proteoforms, protein networks, extracellualr vesicles, circulating antibodies and microsamples. Finally, we provide a prospective view of using the current and emerging proteomics tools in studies of circulating proteins.
- Publisher
- ACS
- Keywords
- Ptm; PeptideAtlas; affinity; biomarker discovery; blood; extracellular vesicle; mass spectrometry; microsampling; plasma; serum
- Research Division(s)
- Advanced Technology And Biology
- PubMed ID
- 39479990
- Publisher's Version
- https://doi.org/10.1021/acs.jproteome.4c00586
- Open Access at Publisher's Site
https://doi.org/10.1021/acs.jproteome.4c00586- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2024-12-05 11:13:22
Last Modified: 2024-12-05 11:14:13