BASTA, a simple whole-blood assay for measuring β cell antigen-specific CD4(+) T cell responses in type 1 diabetes

Lacorcia, M; Bhattacharjee, P; Foster, A; Hardy, MY; Tye-Din, JA; Karas, JA; Wentworth, JM; Cameron, FJ; Mannering, SI

Author(s): Lacorcia, M; Bhattacharjee, P; Foster, A; Hardy, MY; Tye-Din, JA; Karas, JA; Wentworth, JM; Cameron, FJ; Mannering, SI;
Details: Publication Year 2025-03-19,Volume 17,Issue #790,Page eadt2124
Journal Title: Science Translational Medicine
Abstract: Type 1 diabetes (T1D) is an autoimmune disease where T cells mediate the destruction of the insulin-producing β cells found within the islets of Langerhans in the pancreas. Autoantibodies to β cell antigens are the only tests available to detect β cell autoimmunity. T cell responses to β cell antigens, which are known to cause T1D, can only be measured in research settings because of the complexity of assays and the large blood volumes required. Here, we describe the β cell antigen-specific T cell assay (BASTA). BASTA is a simple whole-blood assay that can detect human CD4(+) T cell responses to β cell antigens by measuring antigen-stimulated interleukin-2 (IL-2) production. BASTA is both more sensitive and specific than the CFSE (carboxyfluorescein diacetate succinimidyl ester)-based proliferation assay. We used BASTA to identify the regions of preproinsulin that stimulated T cell responses specifically in blood from people with T1D. BASTA can be done with as little as 2 to 3 milliliters of blood. We found that effector memory CD4(+) T cells are the primary producers of IL-2 in response to preproinsulin peptides. We then evaluated responses to individual and pooled preproinsulin peptides in a cross-sectional study of pediatric patients: without T1D, without T1D but with a first-degree relative with T1D, or diagnosed with T1D. In contrast with other preproinsulin peptides, full-length C-peptide (PI(33-63)) showed high specificity for T1D [area under the curve (AUC) = 0.86)]. We suggest that BASTA will be a useful tool for monitoring changes in β cell-specific CD4(+) T cell responses both in research and clinical settings.
Keywords: Humans; *Diabetes Mellitus, Type 1/immunology/blood; *CD4-Positive T-Lymphocytes/immunology/metabolism; *Insulin-Secreting Cells/immunology; *Interleukin-2; Insulin; Child; Adolescent; Male; Protein Precursors/blood; Female; Adult; Biological Assay/methods
Research Division(s): Immunology
PubMed ID: 40106580
Publisher's Version: https://doi.org/10.1126/scitranslmed.adt2124
Terms of Use/Rights Notice: Refer to copyright notice on published article.

Creation Date: 2025-04-08 02:51:05

Last Modified: 2025-04-08 03:12:51