LeGO-3D: 3D imaging of lung metastases and vascularisation using light sheet fluorescence microscopy
- Author(s)
- Lewis, SM; Berthelet, J; Whitehead, LW; Rajasekhar, P; El-Saafin, F; Bell, C; Naik, S; Merino, D; Wimmer, VC; Rogers, KL;
- Details
- Publication Year 2025-11-07,Volume 3,Issue #1,Page 58
- Journal Title
- Npj Imaging
- Abstract
- Cancer metastasis involves a complex cascade of events, where cancer cells migrate from their site of origin to secondary sites via the lymphatic and circulatory system. During this process, some cancer subclones will successfully 'seed' at distant organs to generate lethal metastases. Here, we optimised a method for tracking cancer cells in metastatic breast cancer tumours and investigated their complex interplay with the lung vasculature using lentiviral-based optical barcoding (LeGO). Given the regional heterogeneity in lung tissue microenvironments as well as lobar asymmetry, we used light sheet microscopy to perform three-dimensional (3D) imaging of wholemount lung lobes. The results revealed that polychromatic metastases occurred less frequently than monochromatic metastases and were more likely to be located nearer to blood vessels in both spontaneous (i.e. mammary fat pad injections) and experimental (i.e. tail vein injections) mouse assays of metastasis. This 3D imaging and analytic pipeline can provide unique insights about metastatic heterogeneity and dynamics, and represents a new avenue for studying therapeutic response across large volumes of lung tissue.
- Publisher
- Springer Nature
- Research Division(s)
- Advanced Technology and Biology; Immunology
- Publisher's Version
- https://doi.org/10.1038/s44303-025-00111-0
- Open Access at Publisher's Site
https://doi.org/10.1038/s44303-025-00111-0- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2025-11-11 11:22:12
Last Modified: 2025-11-11 11:22:24