ErbB receptor stimulation is required for mouse Colon adenoma organoids to form crypts
- Author(s)
- Tan, CW; Zhu, R; Kane, SR; Au, M; Zhang, X; Hirokawa, Y; Faux, MC; Burgess, AW;
- Journal Title
- Growth Factors
- Publication Type
- Mar 31
- Abstract
- When cultured with Wnt, R-spondin, EGF, Noggin, myofibroblast conditioned medium and Matrigel, crypts from normal mouse colon mucosa form crypt-producing organoids and can be passaged every seven days. Under the same culture and passage conditions, crypts isolated from colon adenomas derived from Apc(min/+) mice typically grow as spheroidal cysts and do not produce crypts. The adenoma organoids require EGF, but not Wnt, R-spondin or Noggin for continuous passaging. However, when mouse colon adenoma spheroids are grown for more than 10 days in the presence of EGF, crypt formation occurs. EGF, EREG, β-cellulin, Neuregulin-1 or AREG are sufficient for initiating crypt formation, however, neuregulin-1 is more potent than the other EGF-family members. EGFR and ErbB2 inhibitors both prevent crypt formation in these adenoma cultures. Either EGFR:ErbB2 or ErbB3:ErbB2 signaling is sufficient to initiate adenoma crypt budding and elongation. ErbB2 inhibitors may provide a therapeutic avenue for ablating colon adenomas.
- Publisher
- Taylor & Francis
- Keywords
- EGF family; Egfr; ErbB2; ErbB3; Murine colon adenoma organoids; crypt initiation
- Research Division(s)
- Bioinformatics and Computational Biology; Personalised Oncology
- PubMed ID
- 41915793
- Publisher's Version
- https://doi.org/10.1080/08977194.2026.2649730
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2026-04-27 02:50:42
Last Modified: 2026-04-27 02:50:51