A guide to laser microdissection-based proteomics
- Author(s)
- Shen, C; Schoffer, K; Williams, C; Tanzer, MC;
- Journal Title
- FEBS Journal
- Publication Type
- Jul 10
- Abstract
- Laser microdissection-based proteomics enables spatially resolved profiling of the proteome within intact tissue. Recent advances in histology, image analysis, laser microdissection and mass spectrometry have markedly increased the capability of this technology and driven the development of a range of powerful workflows. These now extend from regional tissue proteomics to emerging single-cell applications, with subcellular proteome mapping on the horizon. In this review, we examine the current state of laser microdissection (LMD)-based proteomics, outline what these approaches can offer, describe the standard workflow, and discuss which applications and instrumentation are best suited to address specific biological questions. We also highlight the strengths and limitations of current workflows and direct readers to key publications that describe and exemplify their successful implementation.
- Publisher
- Wiley
- Keywords
- Deep Visual Proteomics; FFPE tissue; laser microdissection; mass spectrometry; single‐cell proteomics; spatial proteomics
- Research Division(s)
- Inflammation
- PubMed ID
- 42427344
- Publisher's Version
- https://doi.org/10.1111/febs.70653
- Open Access at Publisher's Site
https://doi.org/10.1111/febs.70653- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2026-07-15 09:14:54
Last Modified: 2026-07-15 09:15:02