Kampo Medicines Modulate Angiogenic, Antioxidant, and Inflammatory Pathways in Human Preclinical Models: Implications for Preeclampsia

Binder, NK; Onda, K; Beard, S; Uchiyama, K; Ohi, C; de Alwis, N; Baird, L; Kaitu&#39;u-Lino, TJ; HIRANO, T; Yamada, H; Sakurai, T; Hannan, NJ

Author(s): Binder, NK; Onda, K; Beard, S; Uchiyama, K; Ohi, C; de Alwis, N; Baird, L; Kaitu'u-Lino, TJ; HIRANO, T; Yamada, H; Sakurai, T; Hannan, NJ;
Details: Publication Year 2026-07-14,Volume 15,Issue #7,Page 877
Journal Title: Antioxidants
Abstract: Preeclampsia is a serious pregnancy complication characterised by maternal vascular dysfunction, placental dysfunction, and organ injury, with no effective treatment currently available. Kampo, a system of Japanese traditional medicine comprising standardised herbal formulations, could target pathophysiological pathways driving preeclampsia. We evaluated the effects of select Kampo formulations on markers of preeclampsia using primary human trophoblasts, placental explants, human umbilical vein endothelial cells (HUVECs), and uterine microvascular endothelial cells (UtMVECs). Twelve formulations were initially screened in HUVECs, and six formulations advanced for further study. TNFα was used to induce endothelial dysfunction, and angiogenic, antioxidant, inflammatory, and vascular dysfunction markers were assessed. Overall, Kampo formulations had minimal effect on sFlt-1 expression and only modest effects on sFlt-1 secretion by primary human trophoblast. In contrast, several formulations consistently increased placental growth factor (PlGF) expression and secretion, upregulated HMOX1 in trophoblasts, and enhanced PlGF secretion from placental explants. In endothelial cells, Kampo treatment partially reversed TNFα-induced dysfunction, demonstrated by reduced VCAM1 expression, and additional endothelial cell type-dependent effects on ET-1 and inflammatory pathways. These findings indicate that selected Kampo formulations modulate key pathways involved in the pathophysiology underpinning preeclampsia and warrant further investigation as potential therapeutic candidates.
Publisher: MDPI
Keywords: angiogenesis; endothelial dysfunction; herbal medicine; oxidative stress; placenta
Research Division(s): Cancer Biology and Stem Cells
PubMed ID: 42510608
Publisher's Version: https://doi.org/10.3390/antiox15070877
Open Access at Publisher's Site: https://doi.org/10.3390/antiox15070877
Terms of Use/Rights Notice: Refer to copyright notice on published article.

Creation Date: 2026-07-30 09:25:11

Last Modified: 2026-07-30 09:25:28