Recurrent mechanisms of biallelic epigenetic inactivation reveal new putative tumour suppressor genes in prostate cancer
- Author(s)
- Kiriy, D; Favero, F; Gerhäuser, C; Heilmann, J; Lutsik, P; Gonzalez, FGR; Locallo, A; Jespersen, JS; Gruber, AJ; Olsen, AV; Hernando, B; Cheng, KC; Pellegrina, D; Macintyre, G; Bova, GS; Brewer, DS; Bristow, RG; Brook, MN; Brors, B; Butler, A; Cancel-Tassin, G; Corcoran, NM; Cussenot, O; Eeles, RA; Gihawi, A; Girma, EG; Gnanapragasam, VJ; Hamid, A; Hayes, VM; He, HH; Hovens, CM; Imada, EL; Jakobsdottir, GM; Jung, CH; Khani, F; Kote-Jarai, Z; Lamy, PP; Leeman, G; Loda, M; Marchionni, L; Molania, R; Papenfuss, AT; Pope, B; Queiroz, LR; Rausch, T; Robinson, B; Sahli, A; Sørensen, KD; Uhrig, S; Wedge, DC; Xu, Y; Yamaguchi, TN; Zanettini, C; Cooper, CS; Schlomm, T; Reimand, J; Weischenfeldt, J;
- Details
- Publication Year 2026-09-09,Volume 17,Issue #1,Page 9646
- Abstract
- The inactivation of tumour suppressor genes is a key step in cancer development, and is usually achieved by homozygous loss. In prostate cancer, however, large genomic regions are often hemizygously lost, which complicates the identification of putative tumour suppressors in these regions. Here, we develop Epi2Hit, an integrative computational method that leverages whole genome sequencing, epigenomic profiling and gene expression to identify biallelic inactivation of tumour suppressor genes involving DNA methylation of promoter and enhancer regions of one allele and genomic loss of the other allele. We apply Epi2Hit to a cohort of 2,021 prostate cancers to discover tumour suppressor genes. In particular, we identify epigenetic biallelic inactivation of ZFHX3 at a recurrence level similar to TP53. Biallelic inactivation of ZFHX3, a transcriptional repressor, leads to upregulation of oncogenes, including MYC and a shorter time to metastasis. Finally, we provide evidence that epigenetic silencing as 2nd hit is particularly enriched in regions with nearby essential genes, precluding homozygous loss.
- Publisher
- Springer Nature
- Keywords
- *Prostatic Neoplasms/genetics/pathology; DNA Methylation; Humans; *Genes, Tumor Suppressor; *Epigenesis, Genetic; Gene Expression Regulation, Neoplastic; Male; *Gene Silencing; Promoter Regions, Genetic; Alleles; Homeodomain Proteins/genetics; Whole Genome Sequencing
- Research Division(s)
- Bioinformatics and Computational Biology
- PubMed ID
- 42716948
- Publisher's Version
- https://doi.org/10.1038/s41467-026-72182-5
- Open Access at Publisher's Site
https://doi.org/10.1038/s41467-026-72182-5- Terms of Use/Rights Notice
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Creation Date: 2026-10-02 08:18:58
Last Modified: 2026-10-02 08:19:08