Thermal proteome profiling identifies new drug targets in Plasmodium falciparum parasites
- Author(s)
- Pazicky, S; Dziekan, JM; Tjia, S; Bopp, S; Wirth, D; Preiser, PR; Bozdech, Z;
- Journal Title
- Molecular & Cellular Proteomics
- Publication Type
- Oct 7
- Abstract
- Spreading resistance to clinically used antimalarial drugs increases the need for identification of new drug targets. Here, we screened 25 antimalarials with known and unknown mode of action to validate old and find new drug targets in P. falciparum. Combining experimental approach by proteome-wide cellular thermal shift assay and computational filtering by molecular docking, we found the drugs to bind to previously known drug targets, validated ACS10 as the target of MMV665915 and discovered new drug targets. Furthermore, we updated the experimental and analytical MS-CETSA pipeline with the inclusion of membrane solubilization step, validated the targets of atovaquone and cipargamin on cell lysates and in intact cells and mapped the parasite response to these antimalarials.
- Publisher
- Elsevier
- Keywords
- Cetsa; Malaria; Tpp; drug
- Research Division(s)
- Infection and Global Health
- PubMed ID
- 42843592
- Publisher's Version
- https://doi.org/10.1016/j.mcpro.2026.101678
- Open Access at Publisher's Site
https://doi.org/10.1016/j.mcpro.2026.101678.- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2026-10-12 01:25:23
Last Modified: 2026-10-12 01:25:31