G-CSF-mobilized peripheral blood progenitor cells: In vitro growth pattern and hematopoietic growth factor receptor profile
- Author(s)
- Roberts, AW; Zaiss, M; Boyd, AW; Nicola, NA;
- Details
- Publication Year 1997-04-01,Volume 25,Issue #4,Page 298-305
- Journal Title
- EXPERIMENTAL HEMATOLOGY
- Publication Type
- Journal Article
- Abstract
- The kinetics of colony formation by granulocyte colony-stimulating factor (G-CSF)-mobilized peripheral blood progenitor cells (PBPCs) were monitored using clone-mapping experiments. Compared with normal resting bone marrow (BM), where the ratio of Day 7:Day 14 granulocyte-macrophage colony-forming cells (GM-CFCs) was 1:0.76-1.9, PB was found to be relatively deficient in progenitor cells with the capacity to form colonies by Day 7 (median ratio Day 7:Day 14 1:21). The most mature Day 7 GM-CFCs, those dispersing or extinguishing before Day 14, were almost absent in PB (<1% of all GM-CFCs) but comprised 77% of Day 7 GM-CFCs and 32% of all GM-CFCs in BM. The expression patterns of high affinity receptors for G-CSF, GM-CSF, stem cell factor (SCF), and the ligand for flk-2 on CD38(hi) and CD38(-/dim) PB CD34(+) cells were determined by binding of I-125-labeled ligand and autoradiography. G-CSF receptor (G-CSFR) expression was detected on approximately 25% of CD38(-/dim) cells (estimated mean 105 receptors per positive cell) and was higher in CD38(hi) cells (approximately 50% positive, with a mean of 227 receptors per cell). GM-CSFR expression was low (approximately 25% of cells positive, mean of 120 receptors per cell) and did not vary with CD38 expression. c-kit (SCFR) and flk-2 were expressed by greater than or equal to 90% and greater than or equal to 80% of CD34(+) cells, respectively. SCF binding per cell was greater in the CD38(hi) population, while flk-2 expression did not vary with CD38 expression. These results confirm the heterogeneity of receptor expression by progenitor cells and imply differential regulation of receptor expression during maturation.
- Publisher
- CARDEN JENNINGS PUBL CO LTD
- Keywords
- COLONY-STIMULATING FACTOR; HIGH-DOSE CHEMOTHERAPY; UMBILICAL-CORD BLOOD; C-KIT RECEPTOR; BONE-MARROW; STEM-CELLS; PROLIFERATIVE CAPACITY; MONOCLONAL-ANTIBODY; HUMAN PROMYELOCYTES; CYTOKINE RECEPTORS
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Creation Date: 1997-04-01 12:00:00