Expression and function of members of the cytokine receptor superfamily on breast cancer cells
- Author(s)
- Douglas, AM; Goss, GA; Sutherland, RL; Hilton, DJ; Berndt, MC; Nicola, NA; Begley, CG;
- Details
- Publication Year 1997-02-13,Volume 14,Issue #6,Page 661-669
- Journal Title
- ONCOGENE
- Publication Type
- Journal Article
- Abstract
- Receptors for the cytokines leukemia inhibitory factor (LIF), interleukin-6 (IL-6), oncostatin M (OSM), ciliary neurotrophic factor (CNTF) and interleukin-11 (IL-11) are members of the structurally conserved hemopoietin receptor superfamily, In addition, they all share the transmembrane signalling protein gp130, In this paper the expression and function of this family of receptors in breast cancer cells was examined, RT-PCR analyses demonstrated that gp130 was expressed in 12/12 breast cell lines and the specific receptor alpha-chains for IL-6, LIF, IL-11 and CNTF were expressed in the majority of these cell lines, This was in contrast to other hemopoitin receptors, Examination of 50 clinical samples of malignant breast tissue by RT-PCR showed a similar pattern of expression of gp130 associated receptors, Treatment of breast cancer cell lines with OSM resulted in changes in cellular morphology. Cellular proliferation was inhibited following exposure to OSM (3/4 cell lines), IL-11 (2/4 cell lines), and by IL-6 and LIF (1/4 cell lines), Cell surface binding of LIF and OSM was also documented, The expression of these receptors in 12/12 cell lines and greater than 95% of clinical samples suggests that these molecules may be important in regulating the growth of breast cells.
- Publisher
- STOCKTON PRESS
- Keywords
- LEUKEMIA INHIBITORY FACTOR; COLONY-STIMULATING FACTOR; MAMMARY EPITHELIAL-CELLS; IL-6 SIGNAL TRANSDUCER; CARCINOMA CELLS; PROLACTIN RECEPTORS; MESSENGER-RNAS; EGF RECEPTOR; GM-CSF; INTERLEUKIN-6
- Publisher's Version
- https://doi.org/10.1038/sj.onc.1200882
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 1997-02-13 12:00:00