Identification of a second murine interleukin-11 receptor alpha-chain gene (IL11Ra2) with a restricted pattern of expression
Details
Publication Year 1997-03-15,Volume 40,Issue #3,Page 387-394
Journal Title
GENOMICS
Publication Type
Journal Article
Abstract
The interleukin-ll receptor alpha-chain, a member of the hematopoietin receptor superfamily, forms, together with gp130, a functional high-affinity receptor complex for interleukin 11. We, and others, reported the cloning of the murine interleukin 11 receptor alpha-chain cDNA (IL11Ra) and recently described the structure of the IL11Ra locus. We also described the presence of a second IL11Ra-like locus in some mouse strains. In this study we report that the second locus, designated IL11Ra2, encodes an mRNA species. The transcript was 99% identical to the IL11Ra transcript in the coding and 3'-untranslated region, but had a different 5'-untranslated region. The complete genomic organization of the IL11Ra2 locus is presented, and the two loci are shown to be located on a 200-kb NaeI genomic fragment. Comparison of the expression pattern of the IL11Ra and IL11Ra2 genes using an RT-PCR restriction fragment length polymorphism strategy revealed that while the expression of IL11Ra was widespread, expression of IL11Ra2 was restricted to testis, lymph node, and thymus. (C) 1997 Academic Press.
Publisher
ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS
Keywords
COLONY-STIMULATING FACTOR; MOLECULAR-CLONING; HEMATOPOIETIC CYTOKINE; INHIBITORY FACTOR; PROGENITOR CELLS; FAMILY; SUPERFAMILY; EVOLUTION; INVITRO; GP130
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Creation Date: 1997-03-15 12:00:00
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