The gene for the human interleukin-11 receptor alpha chain locus is highly homologous to the murine gene and contains alternatively spliced first exons
- Author(s)
- Nandurkar, HH; Robb, L; Nicholl, JK; Hilton, DJ; Sutherland, GR; Begley, CG;
- Details
- Publication Year 1997-05,Volume 29,Issue #5,Page 753-766
- Journal Title
- INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
- Publication Type
- Journal Article
- Abstract
- The gene for the murine interleukin-ll receptor a chain (mIL-11R alpha) contains two loci (1 and 2), of which locus 2 is restricted to only some mouse strains. Two alternatively spliced exons (la and Ib) encode the 5' untranslated region (5'UTR) of the murine locus 1. We have characterized the gene for the human interleukin-ll receptor a chain locus (hIL-11R alpha), examined its expression by Northern analysis and determined its chromosomal location by fluorescence in situ hybridization. The presence of exon(s) encoding the 5'UTR and mapping of transcription initiation sites was determined by reverse-transcriptase polymerase chain reaction and 5' rapid amplification of cDNA ends (5'RACE) techniques, The human locus spanned 10 kilobasepairs (kb) and consisted of 14 exons. Two alternatively spliced first exons (la and Ib) encoding the 5'UTR were identified and shared 76 and 73% nucleotide identity with murine exons la and Ib. Multiple transcription start sites were demonstrated for human exon la. The promoter regions of both human exons la and Ib did not display a canonical TATA box. A predominant 1.8 kb transcript for the hIL-11R alpha was present in heart, brain, skeletal muscle, lymph nodes, thymus, appendix, pancreas and foetal liver. The hIL-11R alpha gene was localized to chromosome 9p13. In summary, the hIL-11R alpha gene was highly related to locus 1 of the murine gene and there was no evidence of a second hIL-11R alpha locus. (C) 1997 Elsevier Science Ltd.
- Publisher
- PERGAMON-ELSEVIER SCIENCE LTD
- Keywords
- COLONY-STIMULATING FACTOR; LEUKEMIA-INHIBITORY FACTOR; HUMAN ERYTHROPOIETIN RECEPTOR; CARCINOMA CELL-LINE; SIGNAL-TRANSDUCTION; HEMATOPOIETIC CYTOKINE; MOLECULAR-CLONING; EXPRESSION; TRANSCRIPTION; ORGANIZATION
- Publisher's Version
- https://doi.org/10.1016/S1357-2725(97)00011-3
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- Refer to copyright notice on published article.
Creation Date: 1997-05-01 12:00:00