B cell memory in xid mice is long-lived despite reduced memory B cell frequency
Details
Publication Year 1997-06,Volume 45,Issue #6,Page 655-659
Journal Title
SCANDINAVIAN JOURNAL OF IMMUNOLOGY
Publication Type
Journal Article
Abstract
Brutons tyrosine kinase (Btk) deficient rid mice have a diminished primary T cell dependent immune response, resulting in a reduced memory B cell frequency. Boosting at 35 days post primary immunization, however, generates a normal secondary immune response, indicating a functional memory B cell compartment. The longevity of B cell memory appears to depend on both the presence of antigen and expression of cell survival genes such as bcl-2. Since there is a natural decay in the number of memory B cells over time and since rid B cells have been demonstrated to have reduced Bcl-2 levels, we aimed at determining whether B cell memory of rid mice would be long-lasting. This report demonstrates that memory B cell precursors are detectable in rid mice more than 100 days after primary immunization. Furthermore, a secondary immune response of normal magnitude and kinetics can be generated in rid mice at 150 days after primary immunization indicating that B cell memory is long-lived in rid mice. Thus, although survival of B cell memory is presumably dependent on immunoglobulin (Ig)-mediated interaction with antigen, this interaction does not depend solely on signalling through Btk.
Publisher
BLACKWELL SCIENCE LTD
Keywords
X-LINKED IMMUNODEFICIENCY; BRUTON TYROSINE KINASE; AFFINITY MATURATION; IMMUNE-RESPONSE; BONE-MARROW; ANTIGEN; SELECTION
Terms of Use/Rights Notice
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Creation Date: 1997-06-01 12:00:00
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