Dendritic cell development in culture from thymic precursor cells in the absence of granulocyte/macrophage colony-stimulating factor
- Author(s)
- Saunders, D; Lucas, K; Ismaili, J; Wu, L; Maraskovsky, E; Dunn, A; Shortman, K;
- Details
- Publication Year 1996-12-01,Volume 184,Issue #6,Page 2185-2196
- Journal Title
- JOURNAL OF EXPERIMENTAL MEDICINE
- Publication Type
- Journal Article
- Abstract
- The earliest lymphoid precursor population in the adult mouse thymus had previously been shown to produce not only T cells, but also dendritic cell (DC) progeny on transfer to irradiated recipients. In this study, culture of these isolated thymic precursors with a mixture of cytokines induced them to proliferate and to differentiate to DC, but not to T lineage cells. At least 70% of the individual precursors had the capacity to form DC. The resultant DC were as effective as normal thymic DC in the functional test of T cell stimulation in mixed leukocyte cultures. The cultured DC also expressed high levels of class I and class II major histocompatibility complex, together with CD11c, DEC-205, CD80, and CD86, markers characteristic of mature DC in general. However, they did not express CD8 alpha or BP-1, markers characteristic of normal thymic DC. The optimized mixture of five to seven cytokines required for DC development from these thymic precursors did not include, granulocyte/macrophage colony stimulating factor (GM-CSF), usually required for DC development in culture. The addition of anti-GM-CSF antibody or the use of precursors from GM-CSF-deficient mice did not prevent DC development. Addition of GM-CSF was without effect on DC yield when interleukin (IL) 3 and IL-7 were present, although some stimulation by GM-CSF was noted in their absence. In contrast, DC development was enhanced by addition of the Flt3/Flk2 ligand, in line with the effects of the administration of this cytokine in vivo. The results indicate that the development of a particular lineage of DC, probably those of lymphoid precursor origin, may be independent of the myeloid hormone GM-CSF.
- Publisher
- ROCKEFELLER UNIV PRESS
- Keywords
- TUMOR-NECROSIS-FACTOR; MOUSE BONE-MARROW; LANGERHANS CELLS; PERIPHERAL-BLOOD; CD8 EXPRESSION; FACTOR-ALPHA; T-CELLS; PROGENITORS; DIFFERENTIATION; IDENTIFICATION
- Publisher's Version
- https://doi.org/10.1084/jem.184.6.2185
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 1996-12-01 12:00:00