The xid mutation diminishes memory B cell generation but does not affect somatic hypermutation and selection
- Author(s)
- Ridderstad, A; Nossal, GJV; Tarlinton, DM;
- Details
- Publication Year 1996-10-15,Volume 157,Issue #8,Page 3357-3365
- Journal Title
- JOURNAL OF IMMUNOLOGY
- Publication Type
- Journal Article
- Abstract
- In this study, we examine the relationship between primary and secondary T cell-dependent immune responses using the response of rid mice to the hapten (4-hydroxy-3-nitrophenyl)acetyl (NP) as an experimental model, The reduced serologic primary immune response of rid mice was demonstrated to be caused by a substantially decreased Ab-forming cell (AFC) generation, Furthermore, the germinal center reaction in the primary rid immune response was diminished and the frequency of NP-specific memory B cells prior to secondary immunization was reduced 10-fold, Despite the poor primary response of rid mice, secondary exposure to Ag generated a response that was qualitatively and quantitatively equal to that of wt mice, The number of IgG1 AFCs in spleen and bone marrow increased equally in both groups, as did the proportion of AFCs secreting high affinity Ab in both locations, The extent and distribution of somatic mutations in the V-H genes of rid secondary response B cells was also found to be normal, indicating that the rid gene product is not critical for the processes that result in affinity maturation, Thus, although rid mice generate memory B cells of normal phenotype but at a substantially lower frequency, this does not limit the magnitude of the secondary response, Therefore, our results imply that the reduced memory B cell frequency in xid mice is still above some threshold value necessary for a normal secondary immune response.
- Publisher
- AMER ASSOC IMMUNOLOGISTS
- Keywords
- X-LINKED IMMUNODEFICIENCY; GERMINAL CENTER FORMATION; BRUTON TYROSINE KINASE; CBA-N MICE; IMMUNE-RESPONSE; MOLECULAR CHARACTERIZATION; AFFINITY MATURATION; DEFICIENT MICE; CD40 LIGAND; DEFECT
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Creation Date: 1996-10-15 12:00:00