Molecular cloning of a novel human gene (D11S4896E) at chromosomal region 11p15.5

Author(s): Parker, NJ; Begley, CG; Smith, PJ; Fox, RM;
Details: Publication Year 1996-10-15,Volume 37,Issue #2,Page 253-256
Journal Title: GENOMICS
Publication Type: Journal Article
Abstract: A novel gene (GOK) has been cloned from human chromosome region 11p15.5 that is believed to contain a gene or genes associated with a number of pediatric malignancies, including Wilms tumor. A 4-kb cDNA has been cloned and it encodes a predicted protein of similar to 84 kDa that could be translated in vitro. Computer analysis predicted that the protein had a signal peptide and may contain a transmembrane helix. Restriction mapping by pulsed-field electrophoresis indicates that GOK is located 1.7 kb telomeric of RRM1, and both genes are transcribed in the same direction. GOK displays high evolutionary conservation: cloning and partial sequencing of a mouse genomic clone revealed 90% identity with the human gene at both the nucleotide and the predicted amino acid levels. (C) 1996 Academic Press, Inc.
Publisher: ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS
Keywords: SHORT ARM; TUMOR; MAP; CELLS; EXPRESSION; LOCUS; DNA
Publisher's Version: https://doi.org/10.1006/geno.1996.0553
Terms of Use/Rights Notice: Refer to copyright notice on published article.

Creation Date: 1996-10-15 12:00:00