Dramatic increase in the numbers of functionally mature dendritic cells in Flt3 ligand-treated mice: Multiple dendritic cell subpopulations identified
- Author(s)
- Maraskovsky, E; Brasel, K; Teepe, M; Roux, ER; Lyman, SD; Shortman, K; McKenna, HJ;
- Details
- Publication Year 1996-11-01,Volume 184,Issue #5,Page 1953-1962
- Journal Title
- JOURNAL OF EXPERIMENTAL MEDICINE
- Publication Type
- Journal Article
- Abstract
- Dendritic cells (DC) are the most efficient APC for T cells. The clinical use of DC as vectors for anti-tumor and infectious disease immunotherapy has been limited by their trace levels and accessibility in normal tissue and terminal state of differentiation. In the present study, daily injection of human Flt3 ligand (Flt3L) into mice results in a dramatic numerical increase in cells co-expressing the characteristic DC markers-class II MHC, CD11c, DEC205, and CD86. In contrast, in mice treated with either GM-CSF, GM-CSF plus IL-4, c-kit ligand (c-kitL), or G-CSF, class II+ CD11c(+) cells were not significantly increased. Five distinct DC subpopulations were identified in the spleen of Flt3L-treated mice using CD8 alpha and CD11b expression. These cells exhibited veiled and dendritic processes and were as efficient as rare, mature DC isolated from the spleens of untreated mice at presenting allo-Ag or soluble Ag to T cells, or in priming an Ag-specific T cell response in vivo. Dramatic numerical increases in DC were detected in the bone marrow, gastro-intestinal lymphoid tissue (GALT), liver, lymph nodes, lung, peripheral blood; peritoneal cavity, spleen, and thymus. These results suggest that Flt3L could be used to expand the numbers of functionally mature DC in vivo for use in clinical immunotherapy.
- Publisher
- ROCKEFELLER UNIV PRESS
- Keywords
- COLONY-STIMULATING FACTOR; NECROSIS-FACTOR-ALPHA; MOUSE BONE-MARROW; C-KIT-LIGAND; T-CELLS; B-CELLS; LANGERHANS CELLS; TNF-ALPHA; THYMUS; PROGENITORS
- Publisher's Version
- https://doi.org/10.1084/jem.184.5.1953
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 1996-11-01 12:00:00