Adhesion of malaria-infected red blood cells to chondroitin sulfate A under flow conditions
- Author(s)
- Cooke, BM; Rogerson, SJ; Brown, GV; Coppel, RL;
- Details
- Publication Year 1996-11-15,Volume 88,Issue #10,Page 4040-4044
- Journal Title
- BLOOD
- Publication Type
- Journal Article
- Abstract
- Adhesion of parasitized red blood cells (PRBCs) to microvascular endothelial cells (ECs) is a distinctive feature of Plasmodium falciparum malaria and isa central event in the development of life-threatening complications such as cerebral malaria. PRBCs adhere to several EC-expressed molecules in vitro, but the relative importance of these interactions in vivo remains unclear. Chondroitin sulfate A (CSA) is the most recent EC surface-associated molecule to be implicated in the adhesive process. Accordingly, we have studied adhesion of PRBCs to GSA in vitro using a parallel-plate flow chamber. Under controlled flow conditions, PRBCs adhered to CSA in a concentration-dependent manner at wall-shear stresses Lap to 0.2 Pa, a value that is within the physiological range for venules. Once adhered, PRBCs remained stationary (rather than rolling) and continued to remain stationary even when the wall-shear stress was raised to supravenular levels. The adhesive interaction was strong and a proportion of adherent PRBCs could withstand detachment at stresses up to 2.5 Pa. Soluble CSA at pharmacological concentrations prevented adhesion of flowing PRBCs in a concentration-dependent manner but failed to reverse established adhesion. Adhesion of PRBCs to CSA could contribute to the pathogenesis of malaria, and soluble CSA may have a useful therapeutic effect. (C) 1996 by The American Society of Hematology.
- Publisher
- W B SAUNDERS CO
- Keywords
- RECOMBINANT HUMAN THROMBOMODULIN; HUMAN CEREBRAL MALARIA; PLASMODIUM-FALCIPARUM; ENDOTHELIAL ACTIVATION; SEQUESTRATION; ERYTHROCYTES; CYTOADHESION; MOLECULE-1; PLACENTA; ROLES
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Creation Date: 1996-11-15 12:00:00