Rescue of an infertile transgenic line by ovarian transplantation
- Author(s)
- Harari, D; Bernard, O; Shaw, J;
- Details
- Publication Year 1997-01-01,Volume 2,Issue #2,Page 143-151
- Journal Title
- TRANSGENICS
- Publication Type
- Journal Article
- Abstract
- Two transgenic mice harbouring a gene encoding the truncated Fibroblast Growth Factor Receptor (FGFR1) and driven by the Glial Fibrillary Acidic Protein promoter were generated in an attempt to block FGF signalling in astrocytes. Both primary transgenic animals, a male and a fe male, were infertile. They failed to produce any progeny after extensive standard mating protocols. Histological analysis revealed that the transgenic male lacked mature spermatocytes and exhibited greatly reduced testes. The transgenic female failed to ovulate in response to a standard superovulation protocol. Her ovaries were removed, divided and transplanted into the bursa of ovariectomised histocompatible and non-histocompatible recipients. These recipients were mated and a large number of transgenic progeny were produced from the histocompatible recipients. It is therefore possible that the block in fertility of the founder female was due to factor(s) extrinsic to the ovaries. However, the infertility of the founder mice was most probably not due to transgene expression because the transgenic progeny were fertile. This study demonstrates the generation of a line of mice from an infertile founder by means of ovarian transplantation and may serve as an example for other transgenic models in which breeding lines could potentially be generated from infertile primary transgenic mice.
- Publisher
- HARWOOD ACAD PUBL GMBH
- Keywords
- GROWTH-FACTOR RECEPTOR; ALPHA GENE-EXPRESSION; TARGETED EXPRESSION; LUTEAL FAILURE; MICE; MOUSE; ASTROCYTES; FGF; MUTANT; DIFFERENTIATION
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- Refer to copyright notice on published article.
Creation Date: 1997-01-01 12:00:00