Role of galectin-1 in the developing mouse olfactory system
- Author(s)
- Puche, AC; Poirier, F; Hair, M; Bartlett, PF; Key, B;
- Details
- Publication Year 1996-10-10,Volume 179,Issue #1,Page 274-287
- Journal Title
- DEVELOPMENTAL BIOLOGY
- Publication Type
- Journal Article
- Abstract
- Primary sensory olfactory neurons reside in a neuroepithelium lining the nasal cavity and project topographically onto the surface of the olfactory bulb, a rostral extension of the telencephalon. Galectin-1, a bivalent galactose-binding vertebrate lectin, is expressed in the developing rodent olfactory system. In the present study, the mouse olfactory neuron cell line 4.4.2 was used to examine the role of galectin-1 in neurite outgrowth in vitro. Recombinant galectin-1 has neurite outgrowth-promoting activity when used as a substrate for 4.4.2 cells. When either galectin-1 or lactose was added to the culture media, the neurite outgrowth-promoting activity was abolished. These results demonstrate that galectin-1 can modulate neurite growth in vitro. The in vivo role of galectin-1 was investigated by examining the topographical organization of the olfactory pathway in mice carrying a null mutation for galectin-1. Using Dolichos biflorus agglutinin as a convenient histochemical marker of a subpopulation of primary sensory olfactory neurons which project topographically to the dorsomedial olfactory bulb, we show an aberrant topography of olfactory axons in the null mutants. A subset of primary sensory olfactory axons failed to project to their correct target sites in the caudal olfactory bulb. These data indicate that galectin-1 is involved in the growth and/or guidance of primary sensory olfactory axons between the nasal cavity and the olfactory bulb. This is the first demonstration that a lectin has neurite outgrowth-promoting activity and plays a role in neuronal pathfinding in the mammalian nervous system. (C) 1996 Academic Press, Inc.
- Publisher
- ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS
- Keywords
- GALACTOSIDE-BINDING LECTINS; S-TYPE LECTIN; ENDOGENOUS LECTIN; NERVOUS-SYSTEM; CELL-ADHESION; CARBOHYDRATE-RECOGNITION; SELECTIVE EXPRESSION; ANIMAL LECTINS; MUSCLE LECTIN; BASAL CELLS
- Publisher's Version
- https://doi.org/10.1006/dbio.1996.0257
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- Refer to copyright notice on published article.
Creation Date: 1996-10-10 12:00:00