CSF-1 and interferon-gamma act synergistically to promote differentiation of FDC-P1 cells into macrophages
Details
Publication Year 1998-01-01,Volume 15,Issue #3,Page 159-171
Journal Title
GROWTH FACTORS
Publication Type
Journal Article
Abstract
FDC-P1 cells expressing the wildtype CSF-1 receptor. FDwtfms, differentiate into macrophages during incubation with CSF-1. This response is amplified in the presence of interferon-gamma. Cells expressing the 807F mutant receptor, 807F cells, proliferate in response to CSF-1 and do not differentiate. However, in response to CSF-1 and interferon-gamma they differentiate as well. CSF-1 causes the activation of STAT proteins in FDwtfms cells, but not in 807F cells. Cellular differentiation correlates with a sustained activation of STAT1 and STAT3 in response to interferon-gamma over at least 40 hours. However, interferon-gamma alone did not cause differentiation of cells expressing either receptor. Other defects in response to CSF-1 of the 807F cells, such as lack of PLC gamma 2 activation, were not complemented by co-incubation of the cells with CSF-1 and interferon-gamma. It appears that a combination of signaling pathways are activated by CSF-1 and interferon-gamma which caused the shift of response from proliferation to differentiation in the 807F cells and an enhanced differentiation in the FDwtfms cells.
Publisher
HARWOOD ACAD PUBL GMBH
Keywords
STIMULATING FACTOR-RECEPTOR; PROTEIN-TYROSINE-PHOSPHATASE; STAT3 ACTIVATION; GROWTH-FACTOR; FACTOR-I; TARGETED DISRUPTION; HEMATOPOIETIC-CELLS; SIGNAL-TRANSDUCTION; GENE-EXPRESSION; MYELOID CELLS
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Creation Date: 1998-01-01 12:00:00
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