CSF-1 and interferon-gamma act synergistically to promote differentiation of FDC-P1 cells into macrophages
- Author(s)
- Novak, U; Nicholson, S; Bourette, RP; Rohrschneider, LR; Alexander, W; Paradiso, L;
- Details
- Publication Year 1998-01-01,Volume 15,Issue #3,Page 159-171
- Journal Title
- GROWTH FACTORS
- Publication Type
- Journal Article
- Abstract
- FDC-P1 cells expressing the wildtype CSF-1 receptor. FDwtfms, differentiate into macrophages during incubation with CSF-1. This response is amplified in the presence of interferon-gamma. Cells expressing the 807F mutant receptor, 807F cells, proliferate in response to CSF-1 and do not differentiate. However, in response to CSF-1 and interferon-gamma they differentiate as well. CSF-1 causes the activation of STAT proteins in FDwtfms cells, but not in 807F cells. Cellular differentiation correlates with a sustained activation of STAT1 and STAT3 in response to interferon-gamma over at least 40 hours. However, interferon-gamma alone did not cause differentiation of cells expressing either receptor. Other defects in response to CSF-1 of the 807F cells, such as lack of PLC gamma 2 activation, were not complemented by co-incubation of the cells with CSF-1 and interferon-gamma. It appears that a combination of signaling pathways are activated by CSF-1 and interferon-gamma which caused the shift of response from proliferation to differentiation in the 807F cells and an enhanced differentiation in the FDwtfms cells.
- Publisher
- HARWOOD ACAD PUBL GMBH
- Keywords
- STIMULATING FACTOR-RECEPTOR; PROTEIN-TYROSINE-PHOSPHATASE; STAT3 ACTIVATION; GROWTH-FACTOR; FACTOR-I; TARGETED DISRUPTION; HEMATOPOIETIC-CELLS; SIGNAL-TRANSDUCTION; GENE-EXPRESSION; MYELOID CELLS
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Creation Date: 1998-01-01 12:00:00