Fate of adult neural precursors grafted to adult cortex monitored with a Y-chromosome marker
- Author(s)
- Harvey, AR; Symons, NA; Pollett, MA; Brooker, GJF; Bartlett, PF;
- Details
- Publication Year 1997-12-22,Volume 8,Issue #18,Page 3939-3943
- Journal Title
- NEUROREPORT
- Publication Type
- Journal Article
- Abstract
- FRESHLY dissected and dissociated subventricular zone cells from adult male mice were transplanted into the right cerebral cortex of adult female mice. Host mice were perfused 3 or 7 weeks later. To detect donor male cells, paraffin sections (10 mu m) were hybridized in situ with a Y-chromosome-specific probe. Some hybridized sections were also immunostained with antibodies against glial fibrillary acidic protein (GFAP), NeuN or beta-tubulin. Grafted cells were identified in 80% of host female brains; they were found in both grey and white matter and were located close to the initial injection site. Double-labelling studies showed that, of the surviving cells containing hybridized male nuclei, 5-10% expressed a glial (GFAP) marker and 6-7% expressed neuronal (NeuN or beta-tubulin) markers.
- Publisher
- LIPPINCOTT WILLIAMS & WILKINS
- Keywords
- FIBROBLAST GROWTH-FACTOR; CENTRAL-NERVOUS-SYSTEM; MOUSE-BRAIN; NEURONAL PROGENITORS; IN-VIVO; CELLS; DIFFERENTIATION; MIGRATION; GENERATION; SURVIVAL
- Publisher's Version
- https://doi.org/10.1097/00001756-199712220-00018
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 1997-12-22 12:00:00