Loss of antiviral cytotoxic T-lymphocyte activity during high-level antigen stimulation
- Author(s)
- Rolph, MS; Matthaei, KI; Carbone, FR; Heath, WR; Ramshaw, IA;
- Details
- Publication Year 1998,Volume 11,Issue #4,Page 183-195
- Journal Title
- VIRAL IMMUNOLOGY
- Publication Type
- Journal Article
- Abstract
- High levels of antigenic stimulation can result in deactivation of CD8(+) T cells through a variety of mechanisms, including insufficient T-cell help. In the present study, an adoptive transfer system was established in which ovalbumin (OVA)-specific CD8(+) T cells were transferred to irradiated mice infected with a recombinant vaccinia virus encoding OVA (VV-OVA), Prolonged activation of OVA-specific CD8(+) T cells resulted in a proliferative block in these cells, although cytotoxic function was maintained. Unlike naive and recently activated OVA-specific T cells, these nonproliferative cytotoxic CD8(+) T cells did not have antiviral activity following further transfer to mice infected with VV-OVA, Provision of interleukin-2 (IL-2) at the site of virus infection using a recombinant virus encoding antigen and IL-2, as well as the addition of helper T cells, had no effect on the generation of these dysfunctional T cells, Thus, there was no evidence that lack of T-cell help was responsible for CD8(+) T-cell deactivation in this model.
- Publisher
- MARY ANN LIEBERT INC PUBL
- Keywords
- RECEPTOR TRANSGENIC MICE; VACCINIA VIRUS; CLASS-I; EXTRATHYMIC TOLERANCE; IMMUNE-RESPONSES; CELL RESPONSES; CLONAL ANERGY; INFECTION; INTERLEUKIN-2; APOPTOSIS
- Publisher's Version
- https://doi.org/10.1089/vim.1998.11.183
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 1998-01-01 12:00:00