Immunisation with recombinant AMA-1 protects mice against infection with Plasmodium chabaudi
- Author(s)
- Anders, RF; Crewther, PE; Edwards, S; Margetts, M; Matthew, MLSM; Pollock, B; Pye, D;
- Details
- Publication Year 1998-01,Volume 16,Issue #2-3,Page 240-247
- Journal Title
- VACCINE
- Publication Type
- Journal Article
- Abstract
- The Plasmodium merozoite surface antigen apical membrane antigen-1 (AMA-1) has previously been shown to provide partial protection to Saimiri and rhesus monkeys immunised with recombinant Plasmodium fragile or parasite-derived Plasmodium knowlesi AMA-1, respectively, In the study reported here we have used the Plasmodium chabaudi/mouse model system to extend our-pre-clinical assessment of an AMA-1 vaccine, We describe here the expression of the full-length Plasmodium chabaudi adami AMA-1 and the P. chabaudi adami AMA-1 ectodomain using both baculovirus and Escherichia coli The ectodomain expressed in E. coli, which contained an N-terminal hexa-his tag, was purified by Ni-chelate chromatography and refolded in vitro in the presence of oxidised and reduced glutathione to generate intramolecular disulphide bonds, In a series of vaccine trials, in both inbred and outbred mice, highly significant protection was obtained by immunising with die refolded AMA-1 ectodomain, Protection was shown to correlate with antibody response and was dependent on intact disulphide bonds, Passive transfer of antibodies raised in rabbits against the refolded AMA-1 ectodomain was also protective. In view of this demonstration that E. coli expression of a soluble P. chabaudi AMA-1 domain can generate a vaccine that is effective in mice, we are pursuing a similar approach to generating a vaccine against P. falciparum for testing in human volunteers. (C) 1997 Elsevier Science Ltd. All rights reserved.
- Publisher
- ELSEVIER SCI LTD
- Keywords
- APICAL MEMBRANE ANTIGEN-1; SEQUENCE-ANALYSIS; I AMA-1; FALCIPARUM; KNOWLESI; ANTIBODIES; INHIBIT; VACCINE
- Publisher's Version
- https://doi.org/10.1016/S0264-410X(97)88331-4
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 1998-01-01 12:00:00