Bim: a novel member of the Bcl-2 family that promotes apoptosis
Details
Publication Year 1998-01-15,Volume 17,Issue #2,Page 384-395
Journal Title
EMBO JOURNAL
Publication Type
Journal Article
Abstract
Certain members of the Bcl-2 family inhibit apoptosis while others facilitate this physiological process of cell death, An expression screen for proteins that bind to Bcl-2 yielded a small novel protein, denoted Bim, whose only similarity to any known protein is the short (nine amino acid) BH3 motif shared by most Bcl-2 homologues. Bim provokes apoptosis, and the BH3 region is required for Bcl-2 binding and for most of its cytotoxicity. Like Bcl-2, Bim possesses a hydrophobic C-terminus and localizes to intracytoplasmic membranes, Three Bim isoforms, probably generated by alternative splicing, all induce apoptosis, the shortest being the most potent, Wild-type Bcl-2 associates with Bim in vivo and modulates its death function, whereas Bcl-2 mutants that lack survival function do neither, Significantly, Bcl-x(L) and Bcl-w, the two closest homologues of Bcl-2, also bind to Bim and inhibit its activity, but more distant viral homologues, adenovirus E1B19K and Epstein-Barr virus BHRF-1, can do neither, Hence, Bim appears to act as a 'death ligand' which can only neutralize certain members of the pro-survival Bcl-2 sub-family.
Publisher
OXFORD UNIV PRESS
Keywords
PROGRAMMED CELL-DEATH; CAENORHABDITIS-ELEGANS; PROTEIN IDENTIFICATION; ENDOPLASMIC-RETICULUM; SEQUENCE SIMILARITY; CONSERVED DOMAINS; NUCLEAR-ENVELOPE; HOMOLOG BAK; E1B 19K; B-CELLS
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Creation Date: 1998-01-15 12:00:00
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