Interleukin (IL)-4 is a major regulatory cytokine governing bioactive IL-12 production by mouse and human dendritic cells
- Author(s)
- Hochrein, H; O'Keeffe, M; Luft, T; Vandenabeele, S; Grumont, RJ; Maraskovsky, E; Shortman, K;
- Details
- Publication Year 2000-09-18,Volume 192,Issue #6,Page 823-833
- Journal Title
- JOURNAL OF EXPERIMENTAL MEDICINE
- Publication Type
- Journal Article
- Abstract
- Interleukin (IL)-12 may be secreted as a bioactive T helper type 1 (Th1) cell-inducing heterodimer, as a monomer, or as an antagonistic homodimer. We analyzed the IL-12 produced by mouse splenic dendritic cells (DCs), human thymic DCs, and cultured human monocyte-derived DCs. IL-12 production required both a microbial or T cell-derived stimulus and an appropriate cytokine milieu. The different IL-12 forms were differentially regulated by the cytokines present rather than the stimulus used. IL-4 alone or together with granulocyte/macrophage colony-stimulating factor or interferon gamma effectively enhanced the production of the bioactive heterodimer and selectively reduced the antagonistic homodimer of IL-12. Therefore, IL-4, the major Th2-driving cytokine, provides a negative feedback causing DCs to produce the major Th1-inducing cytokine, bioactive IL-12.
- Publisher
- ROCKEFELLER UNIV PRESS
- Keywords
- BLOOD MONONUCLEAR-CELLS; NECROSIS-FACTOR-ALPHA; T-HELPER CELLS; IN-VIVO; LEISHMANIA-MAJOR; P40 HOMODIMER; STIMULATORY FACTOR; INTERFERON-GAMMA; HUMAN MONOCYTES; MURINE LEISHMANIASIS
- Publisher's Version
- https://doi.org/10.1084/jem.192.6.823
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2000-09-18 12:00:00