Interleukin (IL)-4 is a major regulatory cytokine governing bioactive IL-12 production by mouse and human dendritic cells
Details
Publication Year 2000-09-18,Volume 192,Issue #6,Page 823-833
Journal Title
JOURNAL OF EXPERIMENTAL MEDICINE
Publication Type
Journal Article
Abstract
Interleukin (IL)-12 may be secreted as a bioactive T helper type 1 (Th1) cell-inducing heterodimer, as a monomer, or as an antagonistic homodimer. We analyzed the IL-12 produced by mouse splenic dendritic cells (DCs), human thymic DCs, and cultured human monocyte-derived DCs. IL-12 production required both a microbial or T cell-derived stimulus and an appropriate cytokine milieu. The different IL-12 forms were differentially regulated by the cytokines present rather than the stimulus used. IL-4 alone or together with granulocyte/macrophage colony-stimulating factor or interferon gamma effectively enhanced the production of the bioactive heterodimer and selectively reduced the antagonistic homodimer of IL-12. Therefore, IL-4, the major Th2-driving cytokine, provides a negative feedback causing DCs to produce the major Th1-inducing cytokine, bioactive IL-12.
Publisher
ROCKEFELLER UNIV PRESS
Keywords
BLOOD MONONUCLEAR-CELLS; NECROSIS-FACTOR-ALPHA; T-HELPER CELLS; IN-VIVO; LEISHMANIA-MAJOR; P40 HOMODIMER; STIMULATORY FACTOR; INTERFERON-GAMMA; HUMAN MONOCYTES; MURINE LEISHMANIASIS
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Creation Date: 2000-09-18 12:00:00
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