Chopper, a new death domain of the p75 neurotrophin receptor that mediates rapid neuronal cell death
- Author(s)
- Coulson, EJ; Reid, K; Baca, M; Shipham, KA; Hulett, SM; Kilpatrick, TJ; Bartlett, PF;
- Details
- Publication Year 2000-09-29,Volume 275,Issue #39,Page 30537-30545
- Journal Title
- JOURNAL OF BIOLOGICAL CHEMISTRY
- Publication Type
- Journal Article
- Abstract
- The cytoplasmic juxtamembrane region of the p75 neurotrophin receptor (p75(NTR)) has been found to be necessary and sufficient to initiate neural cell death. The region was named "Chopper" to distinguish it from CD95-like death domains. A 29-amino acid peptide corresponding to the Chopper region induced caspase- and calpain-mediated death in a variety of neural and nonneural cell types and was not inhibited by signaling through Trk (unlike killing by full-length p75(NTR)). Chopper triggered cell death only when bound to the plasma membrane by a lipid anchor, whereas non-anchored Chopper acted in a dominant-negative manner, blocking p75(NTR)-mediated death both in vitro and in vivo. Removal of the ectodomain of p75(NTR) increased the potency of Chopper activity, suggesting that it regulates the association of Chopper with downstream signaling proteins.
- Publisher
- AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
- Keywords
- NERVE GROWTH-FACTOR; NF-KAPPA-B; AMYLOID PRECURSOR PROTEIN; ZINC-FINGER PROTEIN; FACTOR-ALPHA; CASPASE ACTIVATION; SENSORY NEURONS; TRUNCATED FORM; SCHWANN-CELLS; NGF RECEPTOR
- Publisher's Version
- https://doi.org/10.1074/jbc.M005214200
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2000-09-29 12:00:00