Bcl-w is an important determinant of damage-induced apoptosis in epithelia of small and large intestine
- Author(s)
- Pritchard, DM; Print, C; O'Reilly, L; Adams, JM; Potten, CS; Hickman, JA;
- Details
- Publication Year 2000-08-10,Volume 19,Issue #34,Page 3955-3959
- Journal Title
- ONCOGENE
- Publication Type
- Journal Article
- Abstract
- The potential role of the bcl-2 relative bcl-w as a physiological regulator of apoptosis in intestinal epithelia has been investigated, Immunoblots for bcl-w with new monoclonal antibodies revealed that it was expressed in the small intestine and colon, among other murine tissues, as well as in six: human tumour cell lines of epithelial origin, including two colon carcinoma lines, To assess whether bcl-w regulates either spontaneous or damage-induced apoptosis in the small intestine or colon, apoptosis in intestinal crypts of bcl-w -/- and wild-type mice,vas quantified microscopically on a cell positional basis. Spontaneous apoptosis within crypt epithelia was not significantly increased by loss of bcl-w, in either the small intestine or midcolon. However, after treatment with the cytotoxic drug 5-fluorouracil or with gamma-radiation, the bcl-w-null animals exhibited substantially more apoptosis than their wild-type counterparts in both tissues. The greatest enhancement of apoptosis attributable to the absence of bcl-w (up to sixfold) occurred in the small intestine, Hence, bcl-w is an important determinant of damage-induced apoptosis in intestinal epithelia, and unlike bcl-2, which regulates only colonic apoptosis, plays a major role In small intestinal epithelium.
- Publisher
- NATURE PUBLISHING GROUP
- Keywords
- RADIATION-INDUCED APOPTOSIS; GASTROINTESTINAL-TRACT; CELL-SURVIVAL; MICE; EXPRESSION; P53; PROLIFERATION; INHIBITION; FAMILY; CRYPTS
- Publisher's Version
- https://doi.org/10.1038/sj.onc.1203729
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2000-08-10 12:00:00