Hematopoietic-specific genes are not induced during in vitro differentiation of scl-null embryonic stem cells
- Author(s)
- Elefanty, AG; Robb, L; Birner, R; Begley, CG;
- Details
- Publication Year 1997-08-15,Volume 90,Issue #4,Page 1435-1447
- Journal Title
- BLOOD
- Publication Type
- Journal Article
- Abstract
- The helix-loop-helix transcription factor, scl, plays an essential role in hematopoietic development. Embryos in which the gene has been disrupted fail to develop yolk sac erythropoiesis, and scl-null embryonic stem cells do not contribute to hematopoiesis in chimeric mice. To analyze the molecular consequences of scl deficiency, we compared the gene expression profiles of control (wild-type and scl-heterozygous) and scl-null embryonic stem cells differentiated in vitro for up to 12 days. In control and scl-null embryoid bodies the temporal expression pattern of genes associated with the formation of ventral mesoderm, such as Brachyury, bone morphogenetic protein-4, and flk-1, was identical. Similarly, GATA-2, CD34, and c-kit, which are coexpressed in endothelial and hematopoietic lineages, were expressed normally in scl-null embryonic stem cell lines, However, hematopoietic-restricted genes, including the transcription factors GATA-1, EKLF, and PU.1 as well as globin genes and myeloperoxidase, were only expressed in wild-type and scl-heterozygous embryonic stem cells, Indirect immunofluorescence was used to confirm the observations that GATA-1 and globins were only present in control embryoid bodies but that CD34 was found on both control and scl-null embryoid bodies, These data extend the previous gene ablation studies and support a model whereby scl is absolutely required for commitment of a putative hemangioblast to the hematopoietic lineage but that it is dispensable for endothelial differentiation. (C) 1997 by The American Society of Hematology.
- Publisher
- W B SAUNDERS CO
- Keywords
- BONE MORPHOGENETIC PROTEIN-4; TRANSCRIPTION FACTOR GATA-2; RECEPTOR TYROSINE KINASE; ENDOTHELIAL-CELLS; IN-VITRO; ERYTHROID DEVELOPMENT; MESODERM FORMATION; VENTRAL MESODERM; BINDING PROTEIN; MICE LACKING
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Creation Date: 1997-08-15 12:00:00