Antigen-specific CD8(+) T cell subset distribution in lymph nodes draining the site of herpes simplex virus infection
- Author(s)
- Cose, SC; Jones, CM; Wallace, ME; Heath, WR; Carbone, FR;
- Details
- Publication Year 1997-09,Volume 27,Issue #9,Page 2310-2316
- Journal Title
- EUROPEAN JOURNAL OF IMMUNOLOGY
- Publication Type
- Journal Article
- Abstract
- Inoculation with replicating virus leads to an increase in T cell numbers within lymph nodes that drain the site of infection. This increase has been associated with a nonspecific proliferation of bystander cells, with only a minority thought to be directed to the infectious agent. Such an assumption is largely based on precursor cytotoxic T lymphocyte (CTL) estimations using limiting dilution analysis. Recently, studies using more advanced molecular approaches have suggested that such functionally derived precursor frequencies considerably underestimate the proportion of T cells specific for the antigen under investigation. We have defined T cell receptor sequences characteristic of CTL populations directed to a dominant determinant of the herpes simplex virus (HSV) glycoprotein B (gB). In this investigation, we used this receptor signature as a probe to directly monitor changes occurring within lymph nodes draining the sites of active infection with HSV. We found that although lymph node CD8(+) T cell numbers increase as a consequence of HSV infection, the majority of these cells are small resting cells that are not enriched for gB-specific receptors. In contrast, a significant proportion of activated T cells are highly enriched for CTL bearing gB-specific receptors. Our results are therefore consistent with a nonspecific migration of CTL precursors into the lymph nodes draining the site of infection, followed by the activation and proliferation of the antigen-specific subset that normally makes up a small proportion of the naive T cell repertoire.
- Publisher
- VCH PUBLISHERS INC
- Keywords
- IN-VIVO; IMMUNE-RESPONSE; MEMORY; LYMPHOCYTES; RECEPTOR; ACTIVATION; DIVERSITY; PROLIFERATION; REPERTOIRES; INVIVO
- Publisher's Version
- https://doi.org/10.1002/eji.1830270927
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 1997-09-01 12:00:00