Are CD8(+) dendritic cells (DC) veto cells? The role of CD8 on DC in DC development and in the regulation of CD4 and CD8 T cell responses
- Author(s)
- Kronin, V; Vremec, D; Winkel, K; Classon, BJ; Miller, RG; Mak, TW; Shortman, K; Suss, G;
- Details
- Publication Year 1997-07,Volume 9,Issue #7,Page 1061-1064
- Journal Title
- INTERNATIONAL IMMUNOLOGY
- Publication Type
- Journal Article
- Abstract
- The CD8-expressing dendritic cells (DC) present in mouse spleen have been shown to have a regulatory effect on the CD4 and CD8 T cells they activate, restricting subsequent T cell proliferation by either inducing apoptotic T cell death (CD4 T cells) or by limiting endogenous cytokine production (CD8 T cells), To determine the role of the CD8 molecule itself in these regulatory phenomena, the DC from CD8 null mice were studied, The DC marker DEC-205 (NLDC 145) was used as a surrogate marker for CD8, since the expression of these two molecules on splenic DC was closely correlated. DC levels were normal, and the incidence of DEC-205(+) and DEC-205(-) DC was normal in CD8 null mice, indicating that the absence of CD8 did not affect DC development, The proliferative response of T cells to allogeneic DEC-205(+) DC from either CDS-/- or CD8(+/+) mice was similar and was much less than the response to DEC-205(-) DC from these mice, This applied to both the CD4 and the CD8 T cell responses. Thus the lack of the CD8 molecule did not affect the stimulatory or regulatory properties of the DC, The regulatory CD8(+) DEC-205(+) DC therefore differ in that respect from antigen-presenting 'veto' cells, where CD8 itself is involved in transmitting negative signals to the T cells. DEC-205 may prove to be a more pertinent marker of the regulatory DC population.
- Publisher
- OXFORD UNIV PRESS
- Keywords
- I-A; ANTIGENS; SUPPRESSION; LYMPHOCYTES; SPLEEN; MHC
- Publisher's Version
- https://doi.org/10.1093/intimm/9.7.1061
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 1997-07-01 12:00:00