Dendritic cells generated from human blood in granulocyte macrophage-colony stimulating factor and interleukin-7
- Author(s)
- Takahashi, K; Honeyman, MC; Harrison, LC;
- Details
- Publication Year 1997-07,Volume 55,Issue #2,Page 103-116
- Journal Title
- HUMAN IMMUNOLOGY
- Publication Type
- Journal Article
- Abstract
- Dendritic cells (DC), with potentially important clinical applications, have been generated from human peripheral blood monocytes in the presence of GM-CSF and IL-4 (G4 DC). In the present report we show that DC with a novel phenotype can be generated from blood adherent mononuclear cells in the presence of GM-CSF and IL-7 (G7 DC). Adherent cells from PBMC, cultured in GM-CSF (600 U/ml) and IL-7 (6 U/ml), were transformed over 7 days into cells with DC morphology, at a yield of 1.2 - 1.6 X 10(6) per 10(7) PBMC. G7 DC not only expressed class I and class II MHC, CD1a, CD11c, CD23, CD40, CD54, CD58, CD80, CD86 and CD95, like G4 DC, but also CD21, which is the complement receptor type 2, a ligand for CD23 and a receptor for EBV and IFN-alpha. G7 DC were at least one log more effective in the autologous MLR and at lease two logs more effect-ive in the allogeneic MLR, than PBMC. They elicited proliferative responses of CD4 T cells to tetanus toxoid and CD8 T cells ro an EBV peptide, and stronger T-cell cytotoxicity to EBV peptide than G4 DC. Expression of CD21 by G7 DC suggests that IL-7 delivers a distinct signal to DC precursors and that G7 DC may be functionally distinct. (C) American Society for Histocompatibility and Immunogenetics, 1997. Published by Elsevier Science Inc.
- Publisher
- ELSEVIER SCIENCE INC
- Keywords
- TUMOR-NECROSIS-FACTOR; HUMAN BONE-MARROW; C-KIT-LIGAND; PERIPHERAL-BLOOD; T-CELL; LYMPHOCYTES-T; FACTOR-ALPHA; GAMMA-CHAIN; PROGENITORS; EXPRESSION
- Publisher's Version
- https://doi.org/10.1016/S0198-8859(97)00094-3
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 1997-07-01 12:00:00