Local secretion of a chimeric anti-CD4 antibody protects against graft rejection in the NOD mouse
- Author(s)
- McKenzie, AW; Brady, JL; Martin, RM; Georgiou, HM; Lew, AM;
- Details
- Publication Year 2000-04-27,Volume 69,Issue #8,Page 1745-1748
- Journal Title
- TRANSPLANTATION
- Publication Type
- Journal Article
- Abstract
- Background. Engineering a graft to secrete its own immunosuppressive antibodies may minimize the risks associated with current high dose systemic immunosuppression. Methods and Results. A beta cell insulinoma cell line (NIT-1) was transfected with genes encoding a chimeric anti-CD4 antibody. The NIT-1 cells secreted functional chimeric anti-CD4 antibody that bound to the CD4 molecule on mouse thymocytes and inhibited in vitro proliferation of CD4(+ve) T cells. Both test and control transfected cell. lines grew at a similar rate in immunodeficient mice. In immunocompetent NOD mice, NIT-1 cells are normally rejected by a cellular immune response against the SV40 T antigen. Although control transfected NIT-1 cells were rapidly rejected by NOD mice, anti-CD4 secreting NIT-1 cells grew significantly better and were able to form tumors at the site of injection. Conclusions. The local secretion of chimeric anti-CD4 antibody from transfected cells can contribute to graft survival in our transplantation model.
- Publisher
- LIPPINCOTT WILLIAMS & WILKINS
- Keywords
- ISLET ALLOGRAFT SURVIVAL; T-CELL; MONOCLONAL-ANTIBODY; MICE; ANTIGEN; CTLA4IG; GK1.5; XENOGRAFTS; L3T4
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2000-04-27 12:00:00