DEC-205 as a marker of dendritic cells with regulatory effects on CD8 T cell responses
- Author(s)
- Kronin, V; Wu, L; Gong, SC; Nussenzweig, MC; Shortman, K;
- Details
- Publication Year 2000-05,Volume 12,Issue #5,Page 731-735
- Journal Title
- INTERNATIONAL IMMUNOLOGY
- Publication Type
- Journal Article
- Abstract
- We have previously reported that a population of lymphoid-related CD8 alpha(+) DEC-205(+) dendritic cells (DC) from mouse spleen have 'regulatory' effects on the T cells they activate. CD8 T cells produce IL-2 and give a sustained proliferative response to allogeneic CD8 alpha(-) DEC-205(-) splenic DC, but produce little IL-2 and give a limited response to allogeneic CD8(+) DEC-205(+) splenic DC. Although CD8 alpha, and DEC-205 correlate closely among splenic DC, lymph nodes (LN) include a large population of CD8 alpha(low) DEC-205(high) DC. By i.v. transfer of purified thymic early lymphoid precursors into irradiated recipient mice we now demonstrate that these CD8 alpha(low) but DEC-205(high) LN DC can be the progeny of a lymphoid precursor population, apparently corresponding to the CD8 alpha(high) DEC-205(high) DC progeny of the same precursors in spleen and thymus, By culture of the separated, purified DC with allogeneic CD8 T cells we demonstrate that the CD8 alpha(low) DEC-205(high) DC of LN are also functionally equivalent to the CD8 alpha(high) DEC-205(high) DC of spleen. Therefore, DEC-205 but not CD8a serves to segregate functionally distinct DC types in LN. However, DC isolated from the spleens of genetically manipulated DEC-205(null) mice and separated on the basis of CD8 alpha expression have a similar capacity to stimulate CD8 T cells as their heterozygous littermate controls, with the CD8 alpha(+) but now DEC-205(null) DC still giving restricted responses. In conclusion, high expression of DEC-205 appears to be a good marker of the lymphoid-related regulatory type of DC, but DEC-205 itself is not responsible for transmitting negative signals to the T cells.
- Publisher
- OXFORD UNIV PRESS
- Keywords
- PHENOTYPE; RECEPTOR; SUBCLASS; THYMUS; SPLEEN
- Publisher's Version
- https://doi.org/10.1093/intimm/12.5.731
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2000-05-01 12:00:00