Proteomic analysis of colonic crypts from normal, multiple intestinal neoplasia and p53-null mice: A comparison with colonic polyps
- Author(s)
- Cole, AR; Ji, H; Simpson, RJ;
- Details
- Publication Year 2000-05,Volume 21,Issue #9,Page 1772-1781
- Journal Title
- ELECTROPHORESIS
- Publication Type
- Journal Article
- Abstract
- In order to observe cellular changes caused by mutation of the tumor suppressors, APC and p53, we have generated protein expression profiles of mouse colon epithelial cells using two-dimensional electrophoresis (2-DE). Crypts, polyps and stroma were isolated from normal, multiple intestinal neoplasia (MIN) ana p53-null mice, each with a C57Black/6J background, and subjected to 2-DE in two separate pH ranges (pH 3-10 and pH 6-11). No significant differences in protein expression patterns were observed between the normal, MIN and p53-null colon epithelial crypts. However, 64 proteins from the MIN polyps showed a 2-fold or greater difference in intensity that was statistically significant as assessed by the Wilcoxon rank-sum test (p less than or equal to 0.05). Of these, calreticulin, carbonic anhydrase I and a new member of the glutathione-S-transferase theta family of proteins have so far been identified using an in-gel digestion protocol coupled with reversed-phase high performance liquid chromatography (RP-HPLC) ion-trap mass spectrometry. In addition, 38 marker proteins have been identified in a continuing effort to generate a comprehensive 2-DE database of proteins expressed by mouse colon epithelial cells (these databases are available at http:/www. ludwig.edu.au/jpsl/jpSlhome.html.).
- Publisher
- WILEY-V C H VERLAG GMBH
- Keywords
- HUMAN COLORECTAL-CANCER; TUMOR-SUPPRESSOR GENE; CELL-CYCLE CONTROL; WILD-TYPE P53; BETA-CATENIN; CYCLOOXYGENASE-2 LEVELS; SEPARATED PROTEINS; CARBONIC-ANHYDRASE; MESSENGER-RNA; UP-REGULATION
- Publisher's Version
- https://doi.org/10.1002/(SICI)1522-2683(20000501)21:9<1772::AID-ELPS1772>3.3.CO;2-X
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- Refer to copyright notice on published article.
Creation Date: 2000-05-01 12:00:00