Estrogen influences the differentiation, proliferation, and survival of early B-lineage precursors
- Author(s)
- Medina, KL; Strasser, A; Kincade, PW;
- Details
- Publication Year 2000-03-15,Volume 95,Issue #6,Page 2059-2067
- Journal Title
- BLOOD
- Publication Type
- Journal Article
- Abstract
- B lymphocyte production in murine bone marrow is negatively regulated by sex steroids and the aim of this study was to identify early hormone sensitive checkpoints. Estrogen (E2) treatment reduced c mu(+) pre-B cells, a change that occurred concomitantly with decreased Ig gene rearrangements and rag-1 transcripts. Estrogen decreased B lineage precursors in Ig transgenic mice, demonstrating that hormonal regulation is independent of the recombination process. B lineage precursors in Bcl-2 transgenic mice were resistant to estrogen treatment, suggesting that life/death decisions are involved in hormonal regulation. A previously uncharacterized population of CD43(-)c mu(-) lineage precursors was identified in normal, lg transgenic, and RAG(-/-) mice after estrogen treatment, revealing that down-regulation of CD43 can occur independent of Ig heavy chain expression. These cells expressed transcripts for both tdt and bcl-2, characteristics of early B-cell precursors. BrdU incorporation analysis revealed that the mitotic activity of early B-lineage cells is reduced in hormone-treated mice, We conclude that sex steroids modulate the production of B-lineage cells by influencing the differentiation, proliferation, and survival of early B-cell precursors. These findings are informative about mechanisms of hormonal regulation, as well as the significance of some differentiation-related events. (C) 2000 by The American Society of Hematology.
- Publisher
- AMER SOC HEMATOLOGY
- Keywords
- MOUSE BONE-MARROW; SYSTEMIC LUPUS-ERYTHEMATOSUS; IMMUNOGLOBULIN HEAVY-CHAIN; SURROGATE LIGHT-CHAIN; CELL DEVELOPMENT; BCL-2 EXPRESSION; RAG-1-DEFICIENT MICE; LYMPHOID-CELLS; SINGLE CELLS; PRO-B
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Creation Date: 2000-03-15 12:00:00