Identification of a genetic locus modulating splenomegaly induced by granulocyte colony-stimulating factor in mice
- Author(s)
- Roberts, AW; Hasegawa, M; Metcalf, D; Foote, SJ;
- Details
- Publication Year 2000-04,Volume 14,Issue #4,Page 657-661
- Journal Title
- LEUKEMIA
- Publication Type
- Journal Article
- Abstract
- Clinically detectable splenomegaly and splenic rupture are uncommon but potentially life-threatening consequences of G-CSF administration. Increased spleen size in mice injected with G-CSF is a complex genetic trait amenable to investigation in experimental inter-strain crosses by quantitative trait analysis. A quantitative trait locus (QTL) with highly significant linkage (LOD 7.9) for splenomegaly was identified within a 22 centimorgan (cM) region on chromosome 1. Inheritance of a C57BL/6 haplotype in this region was associated with a greater spleen weight. The relevance of this locus was confirmed by analysing the responses of mice congenic for the distal 12 cM of this region (C57BL/6 and C57BL/6.SJL-Ptprc(a) Pep3(b)). Consistent with the QTL effect, mice lacking C57BL/6 alleles in this region had reduced splenomegaly induced by G-CSF. Intriguingly, peripheral brood neutrophilia and progenitor cell mobilisation responses to G-CSF were also significantly influenced.
- Publisher
- STOCKTON PRESS
- Keywords
- BLOOD PROGENITOR CELLS; HEMATOPOIETIC STEM-CELLS; PERIPHERAL-BLOOD; G-CSF; COMPLEX TRAITS; MOBILIZATION; MARROW; TRANSPLANTATION; DISSECTION; FREQUENCY
- Publisher's Version
- https://doi.org/10.1038/sj.leu.2401735
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2000-04-01 12:00:00