The survivin-like C-elegans BIR-1 protein acts with the aurora-like kinase AIR-2 to affect chromosomes and the spindle midzone
- Author(s)
- Speliotes, EK; Uren, A; Vaux, D; Horvitz, HR;
- Details
- Publication Year 2000-08,Volume 6,Issue #2,Page 211-223
- Journal Title
- MOLECULAR CELL
- Publication Type
- Journal Article
- Abstract
- Baculoviral IAP repeat proteins (BIRPs) may affect cell death, cell division, and tumorigenesis. The C. elegans BIRP BIR-l was localized to chromosomes and to the spindle midzone. Embryos and fertilized oocytes lacking BIR-1 had defects in chromosome behavior, spindle midzone formation, and cytokinesis. We observed indistinguishable defects in fertilized oocytes and embryos lacking the Aurora-like kinase AIR-2. AIR-2 was not present on chromosomes in the absence of BIR-I. Histone H3 phosphorylation and HCP-1 staining, which marks kinetochores, were reduced in the absence of either BIR-1 or AIR-2. We propose that BIR-l localizes AIR-2 to chromosomes and perhaps to the spindle midzone, where AIR-2 phosphorylates proteins that affect chromosome behavior and spindle midzone organization. The human BIRP survivin, which is upregulated in tumors, could partially substitute for BIR-1 in C. elegans. Deregulation of bir-l promotes changes in ploidy, suggesting that similar deregulation of mammalian BIRPs may contribute to tumorigenesis.
- Publisher
- CELL PRESS
- Keywords
- CAENORHABDITIS-ELEGANS; CELL-DIVISION; GENE-PRODUCT; APOPTOSIS; CYTOKINESIS; SEGREGATION; INHIBITOR; YEAST; PHOSPHORYLATION; CENTROMERE
- Publisher's Version
- https://doi.org/10.1016/S1097-2765(00)00023-X
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2000-08-01 12:00:00