Identification of DIABLO, a mammalian protein that promotes apoptosis by binding to and antagonizing IAP proteins
Details
Publication Year 2000-07-07, Volume 102, Issue #1, Page 43-53
Journal Title
CELL
Publication Type
Journal Article
Abstract
To identify proteins that bind mammalian IAP homolog A (MIHA, also known as XIAP), we used coimmunoprecipitation and 2D immobilized pH gradient/SDS PAGE, followed by electrospray ionization tandem mass spectrometry. DIABLO (direct IAP binding protein with low pi) is a novel protein that can bind MIHA and can also interact with MIHB and MIHC and the baculoviral IAP, OpIAP. The N-terminally processed, IAP-interacting form of DIABLO is concentrated in membrane fractions in healthy cells but released into the MIHA-containing cytosolic fractions upon UV irradiation. As transfection of cells with DIABLO was able to counter the protection afforded by MIHA against UV irradiation, DIABLO may promote apoptosis by binding to IAPs and preventing them from inhibiting caspases.
Publisher
CELL PRESS
Keywords
PROGRAMMED CELL-DEATH; BCL-X-L; DROSOPHILA CASPASE; INHIBIT APOPTOSIS; SIGNALING COMPLEX; BACULOVIRUS IAP; CYTOCHROME-C; GENE; APAF-1; ACTIVATION
Rights Notice
Refer to copyright notice on published article.


Creation Date: 2000-07-07 12:00:00
Last Modified: 0001-01-01 12:00:00
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