Suppressor of cytokine signaling-3 preferentially binds to the SHP-2-binding site on the shared cytokine receptor subunit gp130

Nicholson, SE; De Souza, D; Fabri, LJ; Corbin, J; Willson, TA; Zhang, JG; Silva, A; Asimakis, M; Farley, A; Nash, AD; Metcalf, D; Hilton, DJ; Nicola, NA; Baca, M

Author(s): Nicholson, SE; De Souza, D; Fabri, LJ; Corbin, J; Willson, TA; Zhang, JG; Silva, A; Asimakis, M; Farley, A; Nash, AD; Metcalf, D; Hilton, DJ; Nicola, NA; Baca, M;
Details: Publication Year 2000-06-06,Volume 97,Issue #12,Page 6493-6498
Journal Title: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Publication Type: Journal Article
Abstract: Suppressor of cytokine signaling-3 (SOCS-3) is one member of a family of intracellular inhibitors of signaling pathways initiated by cytokines that use, among others, the common receptor subunit gp130. The SH2 domain of SOCS-3 has been shown to be essential for this inhibitory activity, and we have used a quantitative binding analysis of SOCS-3 to synthetic phosphopeptides to map the potential sites of interaction of SOCS-3 with different components of the gp130 signaling pathway. The only high-affinity ligand found corresponded to the region of gp130 centered around phosphotyrosine-757 (pY757), previously shown to be a docking site for the tyrosine phosphatase sHP-2. By contrast, phosphopeptides corresponding to other regions within gp130, Janus kinase, or signal transducer and activator of transcription proteins bound to SOCS-3 with weak or undetectable affinity. The significance of pY757 in gp130 as a biologically relevant SOCS-3 docking site was investigated by using transfected 2937 fibroblasts. Although SOCs-3 inhibited signaling in cells transfected with a chimeric receptor containing the wild-type gp130 intracellular domain, inhibition was considerably impaired for a receptor carrying a Y-->F point mutation at residue 757. Taken together, these data suggest that the mechanism by which SOCS-3 inhibits the gp130 signaling pathway depends on recruitment to the phosphorylated gp130 receptor, and that some of the negative regulatory roles previously attributed to the phosphatase SHP-2 might in fact be caused by the action of SOCS-3.
Publisher: NATL ACAD SCIENCES
Keywords: CILIARY NEUROTROPHIC FACTOR; JANUS TYROSINE KINASE; TERMINAL SH2 DOMAIN; SOCS-BOX MOTIF; GENE-EXPRESSION; PROTEIN; ACTIVATION; TRANSDUCTION; PHOSPHATASE; INHIBITION
Publisher's Version: https://doi.org/10.1073/pnas.100135197
Terms of Use/Rights Notice: Refer to copyright notice on published article.

Creation Date: 2000-06-06 12:00:00