Transgenic overexpression of human Bcl-2 in islet beta cells inhibits apoptosis but does not prevent autoimmune destruction
Details
Publication Year 2000-01,Volume 12,Issue #1,Page 9-17
Journal Title
INTERNATIONAL IMMUNOLOGY
Publication Type
Journal Article
Abstract
Insulin-dependent diabetes mellitus results when > 90% of the insulin-producing beta cells in the pancreatic islets are killed as a result of autoimmune attack by T cells. During the progression to diabetes, islet beta cells die as a result of different insults from the immune system, Agents such as perforin and granzymes, CD95 ligand and tumor necrosis factor-alpha, or cytokines and free-radicals have all been shown to cause beta cell apoptosis. The anti-apoptotic protein, Bcl-2, might protect against some of these stimuli. We have therefore generated transgenic mice expressing human Bcl-2 in their islet beta cells. Although Bcl-2 was able to prevent apoptosis induced by cytotoxic agents against beta cells in vitro, Bcl-2 alone could not prevent or ameliorate cytotoxic or autoimmune beta cell damage in vivo.
Publisher
OXFORD UNIV PRESS
Keywords
DEPENDENT DIABETES-MELLITUS; ANTI-FAS ANTIBODY; SELF-ANTIGENS; CYTOCHROME-C; CROSS-PRESENTATION; NITRIC-OXIDE; IFN-GAMMA; T-CELLS; DEATH; MICE
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Creation Date: 2000-01-01 12:00:00
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