Endogenous IGF-1 regulates the neuronal differentiation of adult stem cells
- Author(s)
- Brooker, GJF; Kalloniatis, M; Russo, VC; Murphy, M; Werther, GA; Bartlett, FF;
- Details
- Publication Year 2000-02-01,Volume 59,Issue #3,Page 332-341
- Journal Title
- JOURNAL OF NEUROSCIENCE RESEARCH
- Publication Type
- Journal Article
- Abstract
- Stem cells from the adult forebrain of mice were stimulated to form clones in vitro using fibroblast growth factor-2 (FGF-2). At concentrations above 10 ng/ml of FGF-2,very few clones gave rise to neurons; however, if FGF-2 was removed after 5 days, 20-30% of clones subsequently gave rise to neurons. The number of neuron-containing clones and the number of neurons per clone was significantly enhanced, if insulin-like growth factor (IGF)-1 or heparin were added subsequent to FGF-2 removal. The spontaneous production of neurons after FGF-2 removal was shown to be due to endogenous IGF-I, since antibodies to IGF-1 and an IGF-1 binding protein totally inhibited neuronal production. Similarly, these reagents also abrogated the neuron-promoting effects of heparin. Thus, it appears that endogenous IGF-1 may be a major regulator of stem cell differentiation into neurons. Furthermore, it was found that high levels of IGF-1 or insulin promoted the maturation and affected the neurotransmitter phenotype of the neurons generated. (C) 2009 Wiley-Liss, Inc.
- Publisher
- WILEY-LISS
- Keywords
- GROWTH-FACTOR-I; NEONATAL SUBVENTRICULAR ZONE; NEUROTROPHIC FACTOR PROMOTES; CENTRAL-NERVOUS-SYSTEM; OLFACTORY-BULB; MOUSE BRAIN; NEURAL PRECURSORS; PROGENITOR CELLS; GENE-EXPRESSION; TRANSGENIC MICE
- Publisher's Version
- https://doi.org/10.1002/(SICI)1097-4547(20000201)59:3<332::AID-JNR6>3.0.CO;2-2
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2000-02-01 12:00:00