Characterization of lipophosphoglycan from a Ricin-resistant mutant of Leishmania major

Opat, A; Ng, K; Currie, G; Handman, E; Bacic, A

Author(s): Opat, A; Ng, K; Currie, G; Handman, E; Bacic, A;
Details: Publication Year 1996-06,Volume 6,Issue #4,Page 387-397
Journal Title: GLYCOBIOLOGY
Publication Type: Journal Article
Abstract: One of the virulence factors of the protozoan parasite Leishmania major is the surface glycoconjugate, lipophosphoglycan (LPG), A Ricin-resistant mutant of L. major was generated and characterised,vith respect to its virulence in mice and the structure and expression of LPG, The LPG from this mutant (1F6-B5) retained the tripartite structure of wild-type LPG, comprising a glycosylphosphatidylinositol (GPI) anchor linked to a phosphorylated disaccharide backbone terminating in a nonreducing neutral oligosaccharide cap, The structure of the GPI anchor and the major capping oligosaccharide were identical to wild-type LPG, However, there were variations in the number of phosphorylated repeats (PO4-6Gal(beta 1-4)Man(alpha 1-) comprising the backbone of LPG, although the degree of substitution with side branches (approx, 95%) was similar to that of wild-type LPG, Thus, the mutant LPG was shorter in length having, on average, 15 repeat units per molecule compared with 30 in the wild-type LPG, The mutant LPG contained both arabinose (Ara(beta 1-2)[Gal(beta 1-3)-](1,2)) and galactose ([Gal(beta 1-3)-](1-8)) capped side branches linked to the backbone, In contrast to wild-type LPG, the number of arabinose-capped side chains was significantly reduced, and a new population of galactose-capped (Gal(beta 1-3)](5-8)) side branches was present, The level of LPG expression in mutant parasites was approximately one-tenth of the wildtype parasite, The mutant parasites were avirulent in mice, Over a period of 18 months, they did not cause lesions and organisms could not be isolated from the draining lymph nodes.
Publisher: OXFORD UNIV PRESS UNITED KINGDOM
Keywords: CUTANEOUS LEISHMANIASIS; SURFACE GLYCOPROTEIN; GLYCOSYLATED PHOSPHATIDYLINOSITOLS; MONOCLONAL-ANTIBODIES; DISEASE PATTERNS; NUDE-MICE; PROMASTIGOTES; MACROPHAGES; EXPRESSION; DONOVANI
Publisher's Version: https://doi.org/10.1093/glycob/6.4.387
Terms of Use/Rights Notice: Refer to copyright notice on published article.

Creation Date: 1996-06-01 12:00:00