The interaction of macrophage and non-macrophage tropic isolates of HIV-1 with thymic and tonsillar dendritic cells in vitro
- Author(s)
- Cameron, PU; Lowe, MG; Sotzik, F; Coughlan, AF; Crowe, SM; Shortman, K;
- Details
- Publication Year 1996-04-01,Volume 183,Issue #4,Page 1851-1856
- Journal Title
- JOURNAL OF EXPERIMENTAL MEDICINE
- Publication Type
- Journal Article
- Abstract
- Dendritic cells isolated from thymus and tonsil were tested for susceptibility to HIV-1 strains that are tropic for macrophages or for T cell lines. DCs were purified by cell sorting and before infection expressed high levels of CD4 and HLA-DR and lacked markers for T, B, NK cells, or macrophages. Viral entry and revel-se transcription was found after pulsing with strains of HIV-1 that could infect macrophages. During the first 36 h the PCR signals for gag sequences increased in DCs and macrophages. In contrast little if any viral DNA was found after pulsing macrophages or DCs with HIV-1 that was able to infect T cell lilies. DCs pulsed with HIV-1 were able to transmit infection to responding T cells during an allogeneic or superantigen response. Selection for virus able to infect lymphoid DCs and other DCs expressing CD4 and its transfer to T cells during subsequent immune responses may provide a mechanism for the observed predominance of macrophage-tropic HIV-1 after in vivo transmission.
- Publisher
- ROCKEFELLER UNIV PRESS
- Keywords
- HUMAN-IMMUNODEFICIENCY-VIRUS; PERIPHERAL-BLOOD; T-CELLS; INFECTION; TYPE-1; DYSFUNCTION; DEPLETION; SYSTEM
- Publisher's Version
- https://doi.org/10.1084/jem.183.4.1851
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 1996-04-01 12:00:00