Constitutive class I-restricted exogenous presentation of self antigens in vivo
- Author(s)
- Kurts, C; Heath, WR; Carbone, FR; Allison, J; Miller, JFAP; Kosaka, H;
- Details
- Publication Year 1996-09-01,Volume 184,Issue #3,Page 923-930
- Journal Title
- JOURNAL OF EXPERIMENTAL MEDICINE
- Publication Type
- Journal Article
- Abstract
- Ovalbumin (OVA)-specific CD8(+) T cells from the T cell receptor-transgenic line OT-I (OT-I cells) were injected into unirradiated transgenic RIP-mOVA mice, which express a membrane-bound form of OVA (mOVA) in the pancreatic islet beta cells and the renal proximal tubular cells. OT-I cells accumulated in the draining lymph nodes (LN) of the kidneys and pancreas and in no other LN. They displayed an activated phenotype and a proportion entered cell cycle. Unilateral nephrectomy 7-13 d before inoculation of OT-I cells into RIP-mOVA mice allowed the injected T cells to home only to the regional LN of the remaining kidney (and pancreas), but when the operation was performed 4 h before injecting the T cells, homing to the LN of the excised kidney was evident. When the bone marrow of RIP-mOVA mice was replaced with one of a major histocompatibility haplotype incapable of presenting OVA to OT-I cells, no homing or activation was detectable. Therefore, OT-I cells were activated by OVA presented by short-lived antigen-presenting cells of bone marrow origin present in the draining LN of OVA-expressing tissue. These results provide the first evidence that tissue-associated ''self'' antigens can be presented in the context of class I via an exogenous processing pathway. This offers a constitutive mechanism whereby T cells can be primed to antigens that are present in nonlymphoid tissues, which are not normally surveyed by recirculating naive T cells.
- Publisher
- ROCKEFELLER UNIV PRESS
- Keywords
- TOXIC LYMPHOCYTES-T; POSITIVE SELECTION; BEARING THYMOCYTES; CELLS; MOLECULES; PEPTIDES; PROTEIN; PHAGOCYTOSIS; MACROPHAGES; TRANSFERRIN
- Publisher's Version
- https://doi.org/10.1084/jem.184.3.923
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 1996-09-01 12:00:00