Structure and chromosomal localization of the genomic locus encoding the Kiz1 LIM-kinase gene

Author(s): Bernard, O; Burkitt, V; Webb, GC; Bottema, CDK; Nicholl, J; Sutherland, GR; Matthew, P;
Details: Publication Year 1996-08-01,Volume 35,Issue #3,Page 593-596
Journal Title: GENOMICS
Publication Type: Journal Article
Abstract: We have cloned and characterized the mouse gene encoding Kiz1/Limk1, a new member of the zinc finger LIM family that also has a kinase domain. The gene encompasses 25 kb of the mouse genome, and the organization of its 16 exons does not correlate with its functional domains. The promoter region of Kiz1/Limk1 was identified by cloning a 1.06-kb genomic fragment upstream from the first ATC: in a promotorless CAT vector. This construct was demonstrated to drive CAT expression in Jurkat cells. The promoter sequence lacks conventional TATA and CAAT motifs but contains consensus binding sequences for several transcriptional regulators implicated in control of transcription in many different cell types, including Sp1, Ets, and E2A. Analysis of the chromosomal localization of KIZ1/LIMK1 indicates that it lies on human chromosome 17 in the region 17q25 and on mouse Chromosome 5, band G2. (C) 1996 Academic Press, Inc.
Publisher: ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS
Keywords: ZINC-FINGER; EXPRESSION; THREONINE; PROTEIN; SERINE; SYSTEM; FAMILY
Publisher's Version: https://doi.org/10.1006/geno.1996.0403
Terms of Use/Rights Notice: Refer to copyright notice on published article.

Creation Date: 1996-08-01 12:00:00