Plasmodium falciparum: Chloroquine selection of a cloned line and DNA rearrangements
- Author(s)
- Lim, ASY; Cowman, AF;
- Details
- Publication Year 1996-08,Volume 83,Issue #3,Page 283-294
- Journal Title
- EXPERIMENTAL PARASITOLOGY
- Publication Type
- Journal Article
- Abstract
- The chloroquine sensitive Plasmodium falciparum clone, HB3, was selected for growth in increasing amounts of chloroquine. Increases in the size of chromosome 3 were observed with increased chloroquine selection, with the less chloroquine-sensitive parasites possessing the larger forms of the chromosome. Removal of chloroquine selection resulted in a decrease in size of the larger form to the original size and reexposure of these parasites to chloroquine selected again for parasites with a larger chromosome 3. These results suggest that the increase in size of chromosome 3 was associated with the decreased drug sensitivity in the chloroquine selected parasites. A macrorestriction map of chromosome 3 was constructed using the parent HB3 and drug selected lines. The chromosomal size increase was found to have resulted from DNA amplification occurring in 100-kb amplicons. Other isolates of P. falciparum were screened for large size polymorphism in chromosome 3 to determine if chromosome amplification events in this area of the genome also occurred in field isolates. No size changes-that correlated with the amplified region in the chloroquine selected lines were observed. (C) 1996 Academic Press, Inc.
- Publisher
- ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS
- Keywords
- INFECTED HUMAN-ERYTHROCYTES; THYMIDYLATE SYNTHASE GENE; MULTIDRUG-RESISTANCE GENE; HUMAN MALARIA PARASITES; DIHYDROFOLATE-REDUCTASE; PFMDR1 GENE; PYRIMETHAMINE RESISTANCE; POINT MUTATIONS; DRUG-RESISTANCE; AMPLIFICATION
- Publisher's Version
- https://doi.org/10.1006/expr.1996.0076
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- Refer to copyright notice on published article.
Creation Date: 1996-08-01 12:00:00